NxGEN technology has garnered widespread interest by researchers in the gene therapy field worldwide.
April 12th, 2020 - April 12, 2020 – Wright, 2020. Codon Modification and PAMPs in Clinical AAV Vectors: The Tortoise or the Hare? Molecular Therapy, Vol. 28, No. 3.
A recent publication in Molecular Therapy detailed the data from eight gene therapy clinical trials, including variables such as the AAV serotype employed, the CpG dinucleotide content in the open reading frame of the therapeutic gene, the vector production method, the vector dosage and estimated total capsid dose, the immunosuppressive drugs administered, the correlation of AAV gene transfer with a cytotoxic T lymphocyte response (CTL) response, the outcome peak of the therapeutic transgene, and the durability of the transgene. The power of this clinical trial information is that it is the single largest AAV vector clinical data set for a single disease AAV gene therapy for hemophilia B—and allowed researchers to gain a clear insight into the key determinant for clinical success. Among the variables, CpG content was revealed to be the only factor that correlates well with clinical outcome and long-term transgene expression, with unmethylated CpG content in AAV vectors being the key attribute that triggers transgene expression-limiting immune responses in humans. Click here for full article
March 1st, 2020 - March 1, 2020 – NxGEN Vector Solutions was featured in the March 2020 Issue of Genetic Engineering and Biotechnology News (GEN Magazine).
NxGEN Vector Solutions merged last month with ImmunoCurex, a gene therapy company focused on generating programmed death ligand 1 (PD-L1) polypeptides for AAV gene transfer. NxGEN Vector Solutions will gain a second AAV gene therapy platform—an immunosuppressive therapy to treat immune or inflammatory diseases or conditions—and plans to continue to develop the technology. NxGEN Vector Solutions is collaborating with Robert Jarvik, MD, the inventor of the artificial heart and founder of Jarvik Heart, and Douglas McCarty, PhD, senior director of vector development at Pfizer, on this platform. Click here to read more.
February 14th, 2020 - February 14, 2020 – NxGEN Vector Solutions has submitted a scientific abstract to the 23rd Annual Meeting of American Society of Gene and Cellular Therapy, Boston, Massachusetts, May 12-15, 2020.
The title of the abstract is CpG-Motifs within AAV Vectors Trigger Immune Activation upon Hepatic Gene Transfer. The results demonstrate that CpG depletion of AAV vector genomes provide an avenue to improve the clinical outcome of AAV-mediated gene transfer by preventing elimination of transgene product expressing hepatocytes by vector-induced T cell responses.
December 11th, 2019 - December 11, 2019 – NxGEN Vector Solutions CEO, Dr. Susan Faust, is an invited guest speaker at the American Society of Experimental Neurotherapeutics Annual Meeting, which will take place March 2-5, 2020 in Bethesda, MD.
Dr. Faust will present in the symposium entitled Evolution of AAVs for Widespread Gene Delivery to the Central Nervous System. The title of her presentation is The Immune Response to CNS-Directed AAV Gene Therapy
September 1st, 2019 - September 1, 2019 Industry Leaders attend NxGEN Vector Solutions’ Rooftop Cocktail Reception to learn more about NxGEN’s Transformative Technology.
Millions of patients worldwide suffer from diseases and disorders that can be cured with gene therapy: using viruses to introduce new, healthy, genes into the cells of patients.
The challenge lies in the fact that the body views new genes introduced by viruses as potential threats, and the body’s immune system fights off the viruses with the healthy genes.
NxGEN Vector Solutions Transformative Technology solves this problem.
Find out more at our reception.
Open Bar, Hors d’oeuvres, and Catered Buffet Signature cocktail: “End of the Interferon”
April 29th, 2019 - April 29, 2019 NxGEN Vector Solutions is an Exhibitor at the 22 nd Annual American Society of Gene and Cellular Therapy Meeting.
CEO & Founder, Dr. Susan Faust, meets with ASGCT attendees to discuss NxGEN Vector Solutions’ Transformative Technology at the NxGEN Vector Solutions’ Exhibit Booth in 2019.
January 10th, 2019 - Vandamme et al., 2017, Unraveling the Complex Story of Immune Responses to AAV Vectors Trial After Trial, Human Gene Therapy, Volume 28, No. 11.
“The best trade-off one can currently imagine is to engineer rAAV vectors with better transduction efficiency, carrying optimized therapeutic transgenes and with reduced immunogenic profiles (CpG depleted genome, [Faust et al.], inert capsids, contaminant-free batches, minimum amounts of empty capsids, etc.). Such vectors would provide a higher therapeutic index, as they would permit therapeutic efficiency at doses sufficient to bypass pre-existing humoral immunity, but not high enough to trigger deleterious cellular immunity.” Click here for full article
October 25th, 2018 - Raikwar et al., 2018, Neuro-Immuno-Gene- and Genome-Editing-Therapy for Alzheimer’s Disease: Are We There Yet? Journal of Alzheimer’s Disease, Vol. 65.
“It is important to develop suitable strategies to overcome vector directed immune responses. In this regard, CpG-depleted AAV vectors have been shown to evade immune detection [Faust et al.]. In their pioneering studies, Faust et al. initially tested the immunogenicity of AAVrh32.33 in wild type as well as Tlr9 knockout mice. They found that in Tlr9-deficient mice, IFNγ T cell responses toward capsid and transgene antigen were suppressed resulting in minimal cellular infiltrate and stable transgene expression.” Click here for full article
August 24th, 2018 - Hoffman et al., 2013, Covert Warfare Against the Immune System: Decoy Capsids, Stealth Genomes, and Suppressors, Molecular Therapy, Vol. 21 No. 9.
“Faust et al. hypothesized that a vector genome devoid of CpG motifs would be mostly invisible to TLR9. Sure enough, a vector containing such a stealthy genome packaged into the identical capsid failed to elicit CD8+ T-cell responses to a reporter gene, thus directing long-term expression even in wild-type mice with intact TLR9. The same concept applies to less immunogenic serotypes, where already weak responses can be further reduced. Future studies are likely to adapt this concept to therapeutic gene constructs.” Click here for full article
June 4th, 2018 - Sebastian et al. 2015, In vivo Gene Correction of Cystic Fibrosis; Cystic Fibrosis in the Light of New Research (Book): Chapter 15, DOI: 5772/60697.
“Strategies aimed at minimizing adaptive immunity to AAV vectors or reducing the need for repeat administration continue within the field. Removing CpG motifs from AAV vectors or designing hybrid AAV capsids has been shown to reduce innate and adaptive immune responses following intramuscular delivery [Faust et al.].” Click here for the book